Anti-Glucocorticoid Receptor 抗体 - ChIP Grade (ab3579)

製品の概要

  • 製品名Anti-Glucocorticoid Receptor antibody - ChIP Grade
    Glucocorticoid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
  • アプリケーション適用あり: ChIP, Flow Cyt, ICC, IP, WB, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Non Human Primates
    交差が予測される動物種: Rabbit, Guinea pig
  • 免疫原

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 245-259.
    Sequence:

    CKPLILPDTKPKIKD


    (Peptide available as ab5833)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーショ��� Abreviews 特記事項
EMSA Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. See Lerner et al., 2003.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Glucocorticoid Receptor peptide (ab5833). Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.
IHC-Fr Use a concentration of 1 µg/ml. Staining of GR in rat hippocampus results in staining primarily in the nucleus.
ICC/IF 1/40. PubMed: 19202080

ターゲット情報

  • 機能Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 組織特異性Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 関連疾患Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody - ChIP Grade 画像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579) 使用論文

This product has been referenced in:
  • Wang B  et al. Chondrocyte ß-catenin signaling regulates postnatal bone remodeling through modulation of osteoclast formation in a murine model. Arthritis Rheumatol 66:107-20 (2014). ChIP . Read more (PubMed: 24431282) »
  • Jones CL  et al. Loss of TBL1XR1 Disrupts Glucocorticoid Receptor Recruitment to Chromatin and Results in Glucocorticoid Resistance in a B-Lymphoblastic Leukemia Model. J Biol Chem 289:20502-20515 (2014). WB ; Human . Read more (PubMed: 24895125) »

See all 13 Publications for this product

Product Wall

Thank you very much for this extra information, this is very useful indeed for our investigations.

Please do not hesitate to inform me if the replacement antibody gave you satisfactory results or not.

Thank you foryour cooperation.

I am sorry ab3578 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of ab3579. The order number ****. <...

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Thank you for your enquiry and your interest in our products.
This antibody has not been characterized for ChIP in our lab. The ChIP application has been added to the datasheet based on this specific article:
http://www.ncbi.nlm.nih.gov/pubme...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (LNCaP-1F5 Cell line)
Specification LNCaP-1F5 Cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control PSA enhancer ~4 kb upstream of TSS
Negative control Negative control region on Chromosome X not known to be bound by any nuclear receptors.
Username

Abcam user community

Verified customer

投稿 Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP-1F5 Cell line and A549)
Loading amount 40 µg
Specification LNCaP-1F5 Cell line and A549
Treatment Ethanol, 100 nM Dexamethasone, in LNCaP-1F5 and A549 - 24 hours
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: +4°C
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Dr. Biswajyoti Sahu

Verified customer

投稿 Nov 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Neurons)
Loading amount 20 µg
Specification Neurons
Gel Running Conditions Reduced Denaturing (8% PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (skin epidermis)
Total protein in input 500 µg
Specification skin epidermis
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Oct 06 2008

Thank you for your interests in our products. Yes, we have a product (ab3580) that specifically detects GR-alpha from human, mouse and rat tissues and does not detect GR-beta isoform. I have attached a datasheet link to this product for your easy a...

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Thank you for your patience. I have received a reply from the originator of ab3579 ((Glucocorticoid Receptor Antibody)). At this time, we do not have any data regarding the affinity constant of this antibody. I apologize for any inconvenience th...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit polyclonal to Glucocorticoid Receptor (ab3579) is a good selling antibody. It is yet to be tested by IHC using paraffin embedded sectio...

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