Anti-Glucocorticoid Receptor 抗体 (ab55400)

製品の概要

  • 製品名Anti-Glucocorticoid Receptor antibody
    Glucocorticoid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucocorticoid Receptor
  • 特異性ab55400 detects endogenous levels of total Glucocorticoid Receptor protein.
  • アプリケーション適用あり: WB, ELISA, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human Glucocorticoid Receptor around the phosphorylation site of serine 226 (L-L-S-P-L).

  • ポジティブ・コントロール
    • Jurkat cell extract, treated with EGF (200ng/ml, 15mins). IF/ICC: PC12 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/10000.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 組織特異性Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 関連疾患Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody 画像

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab55400) at 1/500 dilution

    Lane 1 : Jurkat cell extract, treated with EGF (200ng/ml, 15mins), without immunizing peptide
    Lane 2 : Jurkat cell extract, treated with EGF (200ng/ml, 15mins), with immunizing peptide


    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • ab55400 immunoprecipitating Glucocorticoid Receptor from Hepa1-6 (murine hepatoma) cells. 1 mg of cell lysate was incubated with the primary antibody and matrix (Protein A) at a concentration of 20 µl/mg lysate for 18 hours at 4°C in cell lysis buffer. Immunoprecipitate was then Western blotted with ab55400 at 1/500.

    See Abreview

  • ICC/IF image of ab55400 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55400, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucocorticoid Receptor antibody (ab55400) 使用論文

ab55400 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 20 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Total protein in input 1000 µg
Specification Hepa1-6 (murine hepatoma) cells
Immuno-precipitation step Protein A
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投稿 May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Loading amount 50 µg
Specification Hepa1-6 (murine hepatoma) cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Apr 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"