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Our Abpromise guarantee covers the use of ab9568 in the following tested applications.
|ELISA||Use at an assay dependent concentration. A permeabilization step is recommended to detect GCR receptors in peripheral blood and bone marrow cells|
|Flow Cyt||1/100 - 1/1000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 86 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
ab9568 staining human primary neuron cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X 100 in PBST (0.1%Tween) for 30 mins at RT prior to blocking with 10% normal donkey serum for 2 hrs at 21°C. The primary antibody was used at a concentration of 5 µg/ml and incubated with the sample for 18 hrs at 4°C. An Alexa Fluor® 546 conjugated donkey anti-mouse antibody was used as the secondary.
Cells were counterstained with MAP2 and Neurofilament -- Green, and Hoecht's - Blue. Image is 40X, 3D deconvolved 10 planes at 5um steps.