製品の概要

  • 製品名Anti-Glucagon Receptor antibody
    Glucagon Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucagon Receptor
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from internal of human Glucagon Receptor

  • ポジティブ・コントロール
    • extracts from COLO205 cells; HeLa cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.Can be blocked with Glucagon Receptor peptide (ab140072).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-Glucagon Receptor antibody 画像

  • All lanes : Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells
    Lane 2 : extracts from COLO205 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel: same, after pre-incubation with the immunizing peptide.
  • IHC image of ab75240 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75240, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucagon Receptor antibody (ab75240) 使用論文

This product has been referenced in:
  • Komurov K  et al. The glucose-deprivation network counteracts lapatinib-induced toxicity in resistant ErbB2-positive breast cancer cells. Mol Syst Biol 8:596 (2012). WB ; Human . Read more (PubMed: 22864381) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization Yes - H2O2
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Sep 27 2016



Unfortunately we do not offer gold conjugation services. We do, however, provide gold conjugated secondaries, like Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (5nm Gold), pre-adsorbed, ab27235

http://www.abcam.com/rab...

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Thank you for contacting us. We have added the blocking peptide for ab75240 to our catalog as ab140072. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"