製品の概要

  • 製品名
    Anti-Glucagon Receptor antibody
    Glucagon Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucagon Receptor
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Glucagon Receptor.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 機能
    This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gcgr antibody
    • GGR antibody
    • GL R antibody
    • GL-R antibody
    • GLR antibody
    • GLR_HUMAN antibody
    • Glucagon receptor antibody
    • Glucagon receptor precursor antibody
    • MGC138246 antibody
    see all

画像

  • All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 25 kDa,51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab109279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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