リコンビナント
RabMAb

Anti-Glucagon 抗体 [EP3070] (ab92517)

製品の概要

  • 製品名
    Anti-Glucagon antibody [EP3070]
    Glucagon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3070] to Glucagon
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HepG2, Human fetal liver, Mouse kidney and Mouse spleen lysates. Human pancreas tissue.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 関連性
      Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
    • 細胞内局在
      Secreted
    • 参照データベース
    • 別名
      • GCG antibody

    画像

    • Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.

      See Abreview

    • ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • Direct ELISA antibody dose-response curve using recombinant anti-Human Glucagon antibody (ab92517) against recombinant human glucagon protein followed Alkaline Phosphatase conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)  secondary antibody.

      Antigen concentration: 1000 ng/ml

      Primary ab concentration: 0-1000 ng/ml

      Secondary ab concentration: 1:2500 dilution

    • All lanes : Anti-Glucagon antibody [EP3070] (ab92517)

      Lane 1 : HepG2 Lysate
      Lane 2 : Human Fetal liver lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen Lysate

      Predicted band size: 21 kDa

    参考文献

    This product has been referenced in:
    • Monazzam A  et al. Increased Expression of GLP-1R in Proliferating Islets of Men1 Mice is Detectable by [68Ga]Ga-DO3A-VS-Cys40-Exendin-4 /PET. Sci Rep 8:748 (2018). IHC ; Mouse . Read more (PubMed: 29335487) »
    • Prabakaran AD  et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29434026) »

    See all 14 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (pancreas)
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 22 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Mar 24 2017

    Application
    ELISA
    Sample
    Human Recombinant protein (Synthetic peptide)
    Specification
    Synthetic peptide
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type
    Indirect
    Username

    Abcam user community

    Verified customer

    投稿 Mar 01 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Sep 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録