製品の概要

  • 製品名Anti-Glucagon antibody (Biotin)
    Glucagon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucagon (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: IP, ELISA, RIA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

    conjugated to KLH via carboxyl group, corresponding to internal sequence amino acids 53-81 of Human Glucagon.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab48287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-Glucagon antibody (Biotin) 画像

  • IHC image of Glucagon staining in THuman normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48287, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucagon antibody (Biotin) (ab48287) 使用論文

ab48287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"