製品の概要

  • 製品名Anti-Glucagon antibody
    Glucagon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucagon
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Glucagon.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human normal pancreas formalin fixed paraffin embedded tissue section.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • GCG antibody

Anti-Glucagon antibody 画像

  • IHC image of Glucagon staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113247, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucagon antibody (ab113247) 使用論文

ab113247 has not yet been referenced specifically in any publications.

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