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Our Abpromise guarantee covers the use of ab113509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.Can be blocked with GLS2 peptide (ab169954).|
|IHC-P||Use a concentration of 5 µg/ml.|
Ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.
Immunocytochemistry/Immunofluorescence analysis of rat liver cells labelling GLS2 with ab113509 at 20 µg/mL.