製品の概要

  • 製品名Anti-GLP1R antibody
    GLP1R 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GLP1R
  • アプリケーション適用あり: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
    交差が予測される動物種: Sheep
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GLP1R.

    (Peptide available as ab39071.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Tissue lysates: Human Brain, Rat Brain, Mouse Brain

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab39072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23827952
IHC-Fr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).Can be blocked with Human GLP1R peptide (ab39071).
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-GLP1R antibody 画像

  • All lanes : Anti-GLP1R antibody (ab39072) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa


    Exposure time : 2 minutes
  • IHC image of ab39072 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39072, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • IHC image of GLP1R staining in rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39072, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GLP1R antibody (ab39072) 使用論文

This product has been referenced in:
  • Willekens SM  et al. Strain Differences Determine the Suitability of Animal Models for Noninvasive In Vivo Beta Cell Mass Determination with Radiolabeled Exendin. Mol Imaging Biol 18:705-14 (2016). Read more (PubMed: 26886298) »
  • Yang Y  et al. Protective Role of PPARdelta in Lipoapoptosis of Pancreatic ß Cells. Lipids 51:1259-1268 (2016). Read more (PubMed: 27631677) »

See all 17 Publications for this product

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Rat Tissue sections (pancreas)
Specification pancreas
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Jan 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Rat Tissue sections (pancreas)
Specification pancreas
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Jan 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE gels)
Sample Human Cell lysate - whole cell (HUVECells)
Specification HUVECells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

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投稿 Aug 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 37°C
Sample Rat Tissue sections (Vagus nerve)
Specification Vagus nerve
Permeabilization Yes - 0.2% tritonX/PBS/Serum
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jun 17 2013

Thank you for contacting us.

I may be misunderstanding your question but I think you are asking about the size of the region on the antibody that binds to GLP1R, also known as the paratope. The number of amino acids that constitute the parat...

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Thank you for your enquiry.

Indeed, the IHC-P image has been updated. The previous image was from lot GR49151-1 and used 5 ug/ul of primary antibody, whereas the current image was from lot GR63040-1 and used only 1 ug/ul of primary. Please r...

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Thank you for contacting us.

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints.
...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step CasBlock from Invitrogene as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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Dr. Miri Stolovich-Rain

Verified customer

投稿 Apr 26 2012

Merci de votre intérêt pour ab39072. A ma connaissance, cet anticorps n’a pas encore été testé en cytométrie en flux et nous n’offrons pas d’échantillon gratuit à des fins de test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"