• 製品名Anti-GLP1 antibody [4F3]
    GLP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4F3] to GLP1
  • 特異性Reacts with all forms of GLP1, including precursor and GLP1(9-37) /GLP1(9-36amide) metabolite. ab23472 cross-reacts with Liraglutide.
  • アプリケーション適用あり: WB, ICC/IF, Sandwich ELISA, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human, Recombinant fragment
    交差が予測される動物種: Mammal
  • 免疫原

    Full length protein corresponding to GLP1. Synthetic GLP-1(7-36)amide coupled to PPD
    Database link: 2641

  • エピトープMid molecular epitope of GLP1.



Our Abpromise guarantee covers the use of ab23472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 4 kDa.
ICC/IF 1/400.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [8G9] to GLP1 (amidated) (ab26278).

We recommend that ab26278 is used as capture antibody. ab26278 binds to the c-terminus and presents the mid-molecular epitope to the ab23472 detection antibody, so it has easy access to it. For the same reason we do not recommend swapping the pair, as the binding of the mid molecular epitope to the coat will not present the c-terminus epitope to the ab26278 antibody in a very favourable manner.

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • 機能Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 組織特異性Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • 配列類似性Belongs to the glucagon family.
  • 翻訳後修飾Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

Anti-GLP1 antibody [4F3] 画像

  • ab23472 staining GLP1 in Mouse GLUTag intestinal L cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 5% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/400 in 5% serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal (1/150) was used as the secondary antibody.

    See Abreview

  • ab23472 (1µg/ml) staining GLP1 in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-GLP1 antibody [4F3] (ab23472) 使用論文

This product has been referenced in:
  • Jiang S  et al. AMPK-dependent regulation of GLP1 expression in L-like cells. J Mol Endocrinol 57:151-60 (2016). Read more (PubMed: 27493247) »
  • Fishbein TM  et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). WB ; Human . Read more (PubMed: 19519821) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (ThermoScientific 4-20% precise tris-glycine)
Sample Rat Tissue lysate - nuclear (Nucleus of the solitary tract and ileum)
Specification Nucleus of the solitary tract and ileum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Mouse Cell (GLUTag intestinal L cell)
Specification GLUTag intestinal L cell
Permeabilization Yes - 0.1% Triton X 100, 10 mins
Fixative Paraformaldehyde

Jeffrey Gagnon

Verified customer

投稿 Sep 13 2013

Gracias por contactarnos.

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Thank you for your call today and for letting us know about the trouble with this antibody.

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