Anti-GLO1 抗体 (ab96032)
Key features and details
- Rabbit polyclonal to GLO1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GLO1 antibody
GLO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GLO1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 171 of GLO1 (NP_006699).
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT and Raji cell lysates IHC-P: ovarian carcinoma tissue ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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IHC-P | (2) |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. -
パスウェイ
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. -
配列類似性
Belongs to the glyoxalase I family. - Information by UniProt
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参照データベース
- Entrez Gene: 2739 Human
- Entrez Gene: 109801 Mouse
- Omim: 138750 Human
- SwissProt: Q04760 Human
- SwissProt: Q9CPU0 Mouse
- Unigene: 268849 Human
- Unigene: 261984 Mouse
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別名
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
see all
画像
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All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE -
Immunohistochemical analysis of GLO1 in paraffin embedded human ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.
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Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab96032 は 19 報の論文で使用されています。
- Smith AJ et al. GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products. BMC Biol 20:68 (2022). PubMed: 35307029
- Oliveira S et al. Improvement of Glycaemia and Endothelial Function by a New Low-Dose Curcuminoid in an Animal Model of Type 2 Diabetes. Int J Mol Sci 23:N/A (2022). PubMed: 35628465
- Huang J et al. FAM201A knockdown inhibits proliferation and invasion of lung adenocarcinoma cells by regulating miR-7515/GLO1 axis. J Cell Physiol 236:5620-5632 (2021). PubMed: 33687075
- Jandova J & Wondrak GT Genomic GLO1 deletion modulates TXNIP expression, glucose metabolism, and redox homeostasis while accelerating human A375 malignant melanoma tumor growth. Redox Biol 39:101838 (2021). PubMed: 33360689
- Rounds L et al. Glyoxalase 1 Expression as a Novel Diagnostic Marker of High-Grade Prostatic Intraepithelial Neoplasia in Prostate Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34298821