製品の概要

  • 製品名Anti-Gliomedin antibody
    Gliomedin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Gliomedin
  • 特異性ab24483 detects a Gliomedin sized band at 59kDa in WB.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Gliomedin.

    (Peptide available as ab25746.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Brain (Mouse), Spinal Cord (Mouse), Brain (Rat) IF/ICC: PC12 cell line

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab24483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC.
  • 細胞内局在Cell Membrane and Cytoplasmic
  • 参照データベース
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Anti-Gliomedin antibody 画像

  • ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,30 kDa. We are unsure as to the identity of these extra bands.
    ab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.

Anti-Gliomedin antibody (ab24483) 使用論文

This product has been referenced in:
  • Kwon HS  et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. J Biol Chem 288:26357-71 (2013). Read more (PubMed: 23897819) »
  • Kawamura N  et al. Anti-neurofascin antibody in patients with combined central and peripheral demyelination. Neurology 81:714-22 (2013). Human . Read more (PubMed: 23884033) »

See all 4 Publications for this product

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