Glibenclamide (Glyburide), K+ channel blocker (ab120267)
Key features and details
- K+ channel blocker
- CAS Number: 10238-21-8
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Glibenclamide (Glyburide), K+ channel blocker -
製品の詳細
K+ channel blocker -
別名
- Glyburide
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生理活性の詳細
Selective blocker of ATP-sensitive (KIR6.x) inward rectifier K+ channels.
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CAS 番号
10238-21-8 -
構造式
製品の特性
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体系名
N-p-[2-(5-Chloro-2-methoxybenzamido)ethyl]benzenesulfonyl-N'-cyclohexylurea -
分子量
494.00 -
分子式
C23H28ClN3O5S -
PubChem 登録番号
3488 -
保存方法
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
O=C(NC1CCCCC1)NS(=O)(=O)c3ccc(CCNC(=O)c2cc(Cl)ccc2OC)cc3 -
由来
Synthetic
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研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120267の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120267, Glibenclamide (Glyburide), K+ channel blocker
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MEF1 cells were incubated at 37°C for 24h with vehicle control (0 µM) and 5 µM of glibenclamide (ab120267) in DMSO. Increased expression of JNK1+JNK2 (phospho T183 + Y185) (ab4821) correlates with an increase in glibenclamide concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab4821at 1/1000 dilution and ab85139 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (3)
ab120267 は 3 報の論文で使用されています。
- Salvati KA et al. AMPK-mediated potentiation of GABAergic signalling drives hypoglycaemia-provoked spike-wave seizures. Brain 145:2332-2346 (2022). PubMed: 35134125
- Wilkinson CM et al. Glibenclamide, a Sur1-Trpm4 antagonist, does not improve outcome after collagenase-induced intracerebral hemorrhage. PLoS One 14:e0215952 (2019). PubMed: 31042750
- Bragança B et al. Ion Fluxes through KCa2 (SK) and Cav1 (L-type) Channels Contribute to Chronoselectivity of Adenosine A1 Receptor-Mediated Actions in Spontaneously Beating Rat Atria. Front Pharmacol 7:45 (2016). PubMed: 27014060