製品の概要

  • 製品名Anti-Gliadin antibody (HRP)
    Gliadin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Gliadin (HRP)
  • 標識HRP
  • 特異性This antibody reacts with Gliadin
  • アプリケーション適用あり: ELISA, Dot blotmore details
  • 種交差性
    交差種: Wheat
  • 免疫原

    Full length native wheat Gliadin protein (purified) and heat treated Full length native wheat Gliadin protein (purified)

  • 特記事項

    The antibody is conjugated to horseradish peroxidase by a two-step glutaraldehyde procedure. The product is purified to remove unconjugated material.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)The fractionation procedure yields primarily the immunoglobulin fraction of antiserum.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Dot blot
  • 追加情報Dot: 1/500.
    A minimum dilution of 1:500 was determined in a direct dot blot using a 2 - 4 µl dot of gliadin at a concentration of 0.25 - 0.5 mg/ml or 1:150 - 1:300 dilution of sample extract.

    ELISA: 1/1000.
    A minimum dilution of 1:1,000 is determined by direct ELISA using gliadin at 5 µg/ml in carbonate/bicarbonate buffer, pH 9.5 as the coating solution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.
    • 細胞内局在Cytoplasmic; major seed storage protein in wheat.
    • 参照データベース
    • 別名
      • prolamin antibody

    Anti-Gliadin antibody (HRP) (ab49713) 使用論文

    ab49713 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"