製品の概要

  • 製品名Anti-Gliadin antibody [14D5]
    Gliadin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14D5] to Gliadin
  • 特異性This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Common Wheat (Triticum aestivum), Barley (Hordeum vulgare)
  • 免疫原

    Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]

    KLQPFPQPELPYPQPQ

    ) coupled to immunogenic carrier protein.

  • エピトープThe epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.
  • 特記事項


    The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
  • ポリ/モノモノクローナル
  • クローン名14D5
  • ミエローマx63-Ag8.653
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

dilution 1/1000

ターゲット情報

  • 関連性Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.
  • 細胞内局在Cytoplasmic; major seed storage protein in wheat.
  • 別名
    • prolamin antibody

Anti-Gliadin antibody [14D5] 画像

  • All lanes : Anti-Gliadin antibody [14D5] (ab36729) at 1/1000 dilution

    Lane 1 : Rye Lysate
    Lane 2 : Barley Lysate
    Lane 3 : Wheat Lysate


    Performed under reducing conditions.

Anti-Gliadin antibody [14D5] (ab36729) 使用論文

ab36729 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"