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Our Abpromise guarantee covers the use of ab6050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml. See Abreview.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 170 kDa. PubMed: 23293081|
Western blot for Gli3 antibody (ab6050) at 1/500 tested on the following tissue lysates:
Lane 1 : Human Brain
Lane 2: Human Lung
Lane 3: Human Spleen
Lane 4: Mouse Brain
Lane 5: Mouse Lung
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 3 minutes
Expected molecular weight: 2 isoforms of Gli3 exist, one is the full length 170-190kDa and the other is a truncated isoform at ~80kDa.
Cell lysates were loaded at 20
NB: This image shows the truncated isoform of Gli3 is detected by ab6050 in human lung (we do not know the identity of the 120-130kDa band). It was not possible to detect Gli3 immunoreactivity by WB in the other lysates tested. This is likely due to low expression/abundance of Gli3 in these tissue lysates. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, makin
ab6050 at 0.625 µg/ml staining human glioblastoma.
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