製品の概要

  • 製品名Anti-GJB6 antibody
    GJB6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GJB6
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human GJB6.

    (Peptide available as ab109645.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Retina tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

  • 機能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 関連疾患Defects in GJB6 are the cause of ectodermal dysplasia type 2 (ED2) [MIM:129500]; also known as Clouston syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED2 is an autosomal dominant condition characterized by atrichosis, nail hypoplasia and deformities, hyperpigmentation of the skin, normal teeth, normal sweat and sebaceous gland function. Palmoplantar hyperkeratosis is a frequent features. Hearing impairment has been detected in few cases of ED2.
    Defects in GJB6 are the cause of deafness autosomal recessive type 1B (DFNB1B) [MIM:612645]. DFNB1B is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in GJB6 are the cause of deafness autosomal dominant type 3B (DFNA3B) [MIM:612643].
  • 配列類似性Belongs to the connexin family. Beta-type (group I) subfamily.
  • 細胞内局在Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Connexin 30 antibody
    • Connexin-30 antibody
    • Cx30 antibody
    • CXB6_HUMAN antibody
    • DFNA3 antibody
    • DFNA3B antibody
    • DFNB1B antibody
    • ECTD2 antibody
    • ectodermal dysplasia 2, hidrotic (Clouston syndrome) antibody
    • ED2 antibody
    • EDH antibody
    • Gap junction beta 6 protein antibody
    • Gap junction beta-6 protein antibody
    • gap junction protein, beta 6 (connexin 30) antibody
    • gap junction protein, beta 6 antibody
    • Gap junction protein, beta 6, 30kDa antibody
    • GJB6 antibody
    • HED antibody
    • HED2 antibody
    • NSRD1 antibody
    see all

Anti-GJB6 antibody 画像

  • Anti-GJB6 antibody (ab97428) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ICC/IF image of ab97428 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97428 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GJB6 antibody (ab97428) 使用論文

ab97428 has not yet been referenced specifically in any publications.

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