製品の概要
-
製品名Anti-GIP antibody
GIP 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to GIP
-
由来種Rabbit
-
アプリケーション適用あり: WB, IHC-Pmore details
-
種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GIP.
-
ポジティブ･コントロール
- This antibody gave a positive signal in Human Pancreas tissue lysate within western blot and human pancreas FFPE tissue sections.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab72167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
-
配列類似性Belongs to the glucagon family.
-
細胞内局在Secreted.
- Information by UniProt
-
参照データベース
- Entrez Gene: 2695 Human
- Omim: 137240 Human
- SwissProt: P09681 Human
- Unigene: 1454 Human
-
別名
- Gastric Inhibitory Peptide antibody
- Gastric inhibitory polypeptide antibody
- Gastric inhibitory polypeptide precursor antibody
see all
画像
-
Western blot - Anti-GIP antibody (ab72167)Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDa (why is the actual band size different from the predicted?)
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of GIP due to the presence of a 21 amino acid signal peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody (ab72167)IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
ab72167 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"