製品の概要

  • 製品名Anti-GIP antibody
    GIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GIP
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GIP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Pancreas tissue lysate within western blot and human pancreas FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • 配列類似性Belongs to the glucagon family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

Anti-GIP antibody 画像

  • Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GIP antibody (ab72167) 使用論文

ab72167 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"