製品の概要

  • 製品名
    Anti-GIP antibody [04]
    GIP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [04] to GIP
  • 特異性
    Ab30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.
  • アプリケーション
    適用あり: IHC-P, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic human GIP coupled to carrier. The immunogen was adsorbed onto Al(OH)3 and emulsified in Freund's incomplete adjuvant.

  • 特記事項


    Fusion partner: SP2/mIL6.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/10000.

This dilution guideline applies to glutaraldehyde linked GIP OA (molar ratio 4:1) coated at 1 mg/mL. With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000.

IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • 配列類似性
    Belongs to the glucagon family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

画像

  • ab30679 at 1/100 staining GIP in human duodenum by IHC-P.

  • ab30679 at 1/100 staining GIP in human small intestine by IHC-P.

プロトコール

参考文献

This product has been referenced in:
  • Lim DM  et al. Difference in protective effects of GIP and GLP-1 on endothelial cells according to cyclic adenosine monophosphate response. Exp Ther Med 13:2558-2564 (2017). Read more (PubMed: 28565879) »
  • Young RL  et al. Disordered control of intestinal sweet taste receptor expression and glucose absorption in type 2 diabetes. Diabetes 62:3532-41 (2013). Human . Read more (PubMed: 23761104) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (gut)
Specification
gut
Fixative
Paraformaldehyde
Permeabilization
Yes - Antigen retrieval
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Goat Sera as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Ms. Bridgette Chia

Verified customer

投稿 Aug 10 2010

Thank you for your enquiry regarding our product ab30679. I am sorry you have had difficulty obtaining satisfactory results from your binding experiments with this antibody. Although this antibody has been tested by ELISA, it has not been tested for th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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