製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GIGYF1
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 402-499 (GIQLSPGVGS SAGPPGDLED DEGLKHLQQE AEKLVASLQD SSLEEEQFTA AMQTQGLRHS AAATALPLSH GAARKWFYKD PQGEIQGPFT TQEMAEWF) of Human GIGYF1.

  • ポジティブ・コントロール
    • Human kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1.
  • 配列類似性
    Belongs to the PERQ family.
    Contains 1 GYF domain.
  • ドメイン
    The GYF domain interacts with GRB10.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDS2 antibody
    • GIGYF1 antibody
    • GRB10-interacting GYF protein 1 antibody
    • GYF1 antibody
    • PERQ amino acid-rich with GYF domain-containing protein 1 antibody
    • PERQ1 antibody
    • PERQ1_HUMAN antibody
    • Postmeiotic segregation increased 2-like 12 antibody
    • PP3360 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab121784 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121784, at 1/150 dilution, staining GIGYF1 in paraffin-embedded Human kidney tissue, by Immunohistochemistry.

参考文献

ab121784 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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