Rabbit polyclonal to GIGYF1
antigen sequence, corresponding to amino acids 402-499 (GIQLSPGVGS SAGPPGDLED DEGLKHLQQE AEKLVASLQD SSLEEEQFTA AMQTQGLRHS AAATALPLSH GAARKWFYKD PQGEIQGPFT TQEMAEWF) of Human GIGYF1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1.
Belongs to the PERQ family.
Contains 1 GYF domain.
The GYF domain interacts with GRB10.
Information by UniProt
- CDS2 antibody
- GIGYF1 antibody
- GRB10-interacting GYF protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-GIGYF1 antibody (ab121784)
Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab121784 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIGYF1 antibody (ab121784)
ab121784, at 1/150 dilution, staining GIGYF1 in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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