製品の概要

  • 製品名Anti-Giantin antibody - Golgi Marker
    Giantin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Giantin - Golgi Marker
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 - 1350 of Human Giantin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in cervival tissue and MCF7 whole cells using immunohistochemistry and immunocytochemistry, respectively.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能May participate in forming intercisternal cross-bridges of the Golgi complex.
  • 細胞内局在Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 372 kDa Golgi complex associated protein antibody
    • 372 kDa Golgi complex-associated protein antibody
    • GCP antibody
    • GCP372 antibody
    • Giantin antibody
    • GOGB1_HUMAN antibody
    • GOLGB1 antibody
    • Golgi autoantigen golgin subfamily a member 1 antibody
    • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody
    • Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody
    • Golgi complex associated protein 372kDa antibody
    • Golgi complex-associated protein 372kDa antibody
    • Golgin B1 antibody
    • Golgin B1, golgi integral membrane protein antibody
    • Golgin subfamily B member 1 antibody
    • GOLIM1 antibody
    • Macrogolgin antibody
    • MGC33154 antibody
    see all

Anti-Giantin antibody - Golgi Marker 画像

  • ICC/IF image of ab80864 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
  • IHC image of ab80864 staining in human cervix carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80864, 0.1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Giantin antibody - Golgi Marker (ab80864) 使用論文

This product has been referenced in:
  • Mousnier A  et al. Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein secretion. J Virol 88:11671-85 (2014). Read more (PubMed: 25100828) »
  • Glotfelty LG  et al. Microtubules are required for efficient epithelial tight junction homeostasis and restoration. Am J Physiol Cell Physiol 307:C245-54 (2014). Read more (PubMed: 24920678) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Permeabilization Yes - 0.2% Triton X-100
Specification WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
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投稿 Oct 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"