製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Giantin
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (NKYIEEMKAQ GGTVLPTEPQ SEEQLSKHDK SSTEEEMEIE KIKHKLQEKE) of Human Giantin (NP_004478).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 376 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May participate in forming intercisternal cross-bridges of the Golgi complex.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 372 kDa Golgi complex associated protein antibody
    • 372 kDa Golgi complex-associated protein antibody
    • GCP antibody
    • GCP372 antibody
    • Giantin antibody
    • GOGB1_HUMAN antibody
    • GOLGB1 antibody
    • Golgi autoantigen golgin subfamily a member 1 antibody
    • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody
    • Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody
    • Golgi complex associated protein 372kDa antibody
    • Golgi complex-associated protein 372kDa antibody
    • Golgin B1 antibody
    • Golgin B1, golgi integral membrane protein antibody
    • Golgin subfamily B member 1 antibody
    • GOLIM1 antibody
    • Macrogolgin antibody
    • MGC33154 antibody
    see all

画像

  • Anti-Giantin antibody (ab93281) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 376 kDa
    Gel concentration 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling Giantin with ab93281 at 5µg/ml.

参考文献

This product has been referenced in:
  • Barretta ML  et al. Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2. Nat Commun 7:11727 (2016). Read more (PubMed: 27242098) »

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ervix (epithelial))
Permeabilization
Yes - 0.2% triton
Specification
ervix (epithelial)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Methanol
Username

Abcam user community

Verified customer

投稿 Jan 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jun 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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