製品の概要

  • 製品名Anti-GHRHR antibody
    GHRHR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GHRHR
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide derived from the internal region of human GHRHR.

  • ポジティブ・コントロール
    • HUVEC cells. Extracts from LOVO cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa. Use BSA for blocking
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 20616039
Flow Cyt Use at an assay dependent concentration. PubMed: 25404316

ターゲット情報

  • 機能Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
  • 組織特異性Pituitary gland.
  • 関連疾患Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH.
  • 配列類似性Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GHRFR antibody
    • GHRH R antibody
    • GHRH receptor antibody
    • Ghrhr antibody
    • GHRHR_HUMAN antibody
    • GHRHRpsv antibody
    • GRF R antibody
    • GRF receptor antibody
    • GRFR antibody
    • Growth hormone releasing factor receptor antibody
    • Growth hormone releasing hormone receptor antibody
    • Growth hormone-releasing factor receptor antibody
    • Growth hormone-releasing hormone receptor antibody
    see all

Anti-GHRHR antibody 画像

  • All lanes : Anti-GHRHR antibody (ab76263) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : >117 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HUVEC cells using ab76263 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of mouse pituitary cells, staining GHRHR with ab76263.

    Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/50) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with an AlexaFluor®488-conjugated anti- rabbit secondary antibody (1/1000) for 1 hour at room temperature.

Anti-GHRHR antibody (ab76263) 使用論文

This product has been referenced in:
  • Kovári B  et al. Differential immunostaining of various types of breast carcinomas for growth hormone-releasing hormone receptor - Apocrine epithelium and carcinomas emerging as uniformly positive. APMIS 122:824-31 (2014). Read more (PubMed: 24479854) »
  • Florea V  et al. Agonists of growth hormone-releasing hormone stimulate self-renewal of cardiac stem cells and promote their survival. Proc Natl Acad Sci U S A 111:17260-5 (2014). Flow Cyt ; Mouse, Rat . Read more (PubMed: 25404316) »

See all 6 Publications for this product

Product Wall

The amino acid range of this antibody is aa350-400 of human GHRHR with accession Q02643(http://www.uniprot.org/uniprot/Q02643). This antibody binds to C-terminal cytoplasmic domain of the protein (based on prediction).



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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"