製品の概要

  • 製品名Anti-GHRHR antibody
    GHRHR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GHRHR
  • 特異性ab28692 recognises GHRH-R.
  • アプリケーション適用あり: WB, ICC, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    RTRAKWTTPSRSAAKVLTSM

    conjugated to KLH, corresponding to amino acids 403-422 of Human GHRHR

  • ポジティブ・コントロール
    • human ovarian carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC 1/3000.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval via the microwave method for 20 minutes in citric acid at pH 6.0.

ターゲット情報

  • 機能Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
  • 組織特異性Pituitary gland.
  • 関連疾患Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH.
  • 配列類似性Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GHRFR antibody
    • GHRH R antibody
    • GHRH receptor antibody
    • Ghrhr antibody
    • GHRHR_HUMAN antibody
    • GHRHRpsv antibody
    • GRF R antibody
    • GRF receptor antibody
    • GRFR antibody
    • Growth hormone releasing factor receptor antibody
    • Growth hormone releasing hormone receptor antibody
    • Growth hormone-releasing factor receptor antibody
    • Growth hormone-releasing hormone receptor antibody
    see all

Anti-GHRHR antibody 画像

  • ab28692 staining Human GHRHR in human ovarian carcinoma samples by immunohistochemistry using paraffin embedded tissue.

  • Anti-GHRHR antibody (ab28692) at 1/500 dilution + LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab28692 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28692, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GHRHR antibody (ab28692) 使用論文

This product has been referenced in:
  • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). WB . Read more (PubMed: 24379381) »
  • Qin YJ  et al. Antagonist of GH-releasing hormone receptors alleviates experimental ocular inflammation. Proc Natl Acad Sci U S A 111:18303-8 (2014). Read more (PubMed: 25489106) »

See all 9 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"