製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab99449 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 23879975

ターゲット情報

  • 機能
    Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
  • 組織特異性
    Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.
  • 配列類似性
    Belongs to the membrane-bound acyltransferase family.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FKSG89 antibody
    • ghrelin o transferas antibody
    • Ghrelin O-acyltransferase antibody
    • GOAT antibody
    • MBOA4_HUMAN antibody
    • Mboat4 antibody
    • Membrane-bound O-acyltransferase domain-containing protein 4 antibody
    • O-acyltransferase (membrane bound) domain containing 4 antibody
    • O-acyltransferase domain-containing protein 4 antibody
    • OACT4 antibody
    see all

画像

  • ab99449 staining Ghrelin O acyltransferase in Human Prostate cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100 in blocking buffer) for overnight at 4°C. An HRP-conjugated secondary was used and shows diffuse cytoplasmic staining.

プロトコール

参考文献

This product has been referenced in:
  • Seim I  et al. Ghrelin O-acyltransferase (GOAT) is expressed in prostate cancer tissues and cell lines and expression is differentially regulated in vitro by ghrelin. Reprod Biol Endocrinol 11:70 (2013). IHC-P ; Human . Read more (PubMed: 23879975) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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