Anti-Ghrelin 抗体 (Biotin) (ab48285)

製品の概要

  • 製品名Anti-Ghrelin antibody (Biotin)
    Ghrelin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ghrelin (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: ELISA, IP, RIA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cat, Dog, Pig, Baboon, Rhesus monkey, Gerbil
  • 免疫原

    Synthetic peptide: H-GSS(octanoyl)FLSPE HQRVQQRKES KKPPAKLQPR-OH conjugated to KLH via carboxyl group, corresponding to internal sequence amino acids 24-51 of Human Ghrelin.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use a concentration of 2 - 8 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • 組織特異性Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • 配列類似性Belongs to the motilin family.
  • 翻訳後修飾O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Anti-Ghrelin antibody (Biotin) 画像

  • IHC image of Ghrelin staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48285, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Ghrelin antibody (Biotin) (ab48285) 使用論文

ab48285 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for contacting Abcam regarding ab48285.


I am sorry that you have been experiencing difficulties with this antibody in your RIA experiments. I understand that you have tested a concentration of 4ug/ml - have you tested any othe...

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Thank you for contacting us.

The supplying lab sent us the following information:

The antibody isrecommended from 2-8 ug/ml for RIA purpose. The efficiency of labeling of their tracer is another factor to determine the concentrati...

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Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and ap...

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ab48285 has not been tested to determine if the antibody will bind Ghrelin whether it has octanoyl or no octanoyl.  Sorry this aspect of ab48285 has not yet been charactarized. If you have any other questions please feel free to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"