Anti-Ghrelin 抗体 (ab104307)
Key features and details
- Goat polyclonal to Ghrelin
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ghrelin antibody
Ghrelin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Ghrelin -
由来種
Goat -
特異性
This antibody is expected to recognise all reported isoforms.
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アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Pig, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Ghrelin aa 100-200 (C terminal).
Database link: Q9UBU3 -
ポジティブ・コントロール
- Human Colon and Human Placenta tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
組織特異性
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
配列類似性
Belongs to the motilin family. -
翻訳後修飾
O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 51738 Human
- Entrez Gene: 58991 Mouse
- Entrez Gene: 59301 Rat
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- SwissProt: Q9EQX0 Mouse
- SwissProt: Q9GKY5 Pig
- SwissProt: Q9QYH7 Rat
see all -
別名
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
画像
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Formalin-fixed, paraffin-embedded Human Colon stained with ab104307 at 2 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Formalin-fixed, paraffin-embedded Human Placenta stained with ab104307 at 2 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab104307 は 2 報の論文で使用されています。
- Nunez-Salces M et al. The Effect of High-Fat Diet-Induced Obesity on the Expression of Nutrient Chemosensors in the Mouse Stomach and the Gastric Ghrelin Cell. Nutrients 12:N/A (2020). PubMed: 32824949
- Fan L et al. Enhancement of the chondrogenic differentiation of mesenchymal stem cells and cartilage repair by ghrelin. J Orthop Res N/A:N/A (2019). PubMed: 30644571