Anti-Ghrelin 抗体 [45] (ab112477)
Key features and details
- Mouse monoclonal [45] to Ghrelin
- Suitable for: ELISA
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Ghrelin antibody [45]
Ghrelin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [45] to Ghrelin -
由来種
Mouse -
特異性
ab112477 binds Human ghrelin and Human des-acyl ghrelin, cross-reacting ~5% with rat ghrelin (determined by sandwich ELISA). -
アプリケーション
適用あり: ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Ghrelin. Synthetic Human Ser3-octanoyl ghrelin-28
Database link: Q9UBU3-1 -
エピトープ
Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 97% PBS, 2.9% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
45 -
アイソタイプ
IgG1 -
軽鎖の種類
lambda -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112477の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
組織特異性
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
配列類似性
Belongs to the motilin family. -
翻訳後修飾
O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 51738 Human
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- Unigene: 590080 Human
- Unigene: 672979 Human
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別名
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab112477 は 2 報の論文で使用されています。
- Kojima M & Kangawa K Ghrelin: structure and function. Physiol Rev 85:495-522 (2005). PubMed: 15788704
- Kojima M et al. Ghrelin: discovery of the natural endogenous ligand for the growth hormone secretagogue receptor. Trends Endocrinol Metab 12:118-22 (2001). PubMed: 11306336