製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GGA3
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide: SPRPGTAACGASDAPL conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3 (BAA09926).

  • ポジティブ・コントロール
    • Human breast tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

ターゲット情報

  • 機能
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • ドメイン
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • 翻訳後修飾
    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.
  • 細胞内局在
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosylation factor binding protein 3 antibody
    • ADP ribosylation factor binding protein GGA 3 antibody
    • ADP ribosylation factor binding protein GGA3 antibody
    • ADP-ribosylation factor-binding protein GGA3 antibody
    • ARF binding protein GGA 3 antibody
    • ARF binding protein GGA3 antibody
    • ARF-binding protein 3 antibody
    • gamma ear-containing antibody
    • GGA 3 antibody
    • GGA3 antibody
    • GGA3_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
    • Golgi localized gamma ear containing ARF binding protein 3 antibody
    • Golgi-localized antibody
    • KIAA0154 antibody
    see all

画像

  • ab117086, at 2.5 µg/ml, staining GGA3 in Formalin-fixed, Paraffin-embedded Human Breast tissue tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

参考文献

ab117086 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab117086.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録