製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GGA3
  • アプリケーション
    適用あり: WB, IHC (PFA fixed)more details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    ADPAPNVPPKESAGNS

    conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.

  • 特記事項
    Expiration date is 1 year from date of opening.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC (PFA fixed) 1/40. PubMed: 17116753

ターゲット情報

  • 機能
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • ドメイン
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • 翻訳後修飾
    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.
  • 細胞内局在
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosylation factor binding protein 3 antibody
    • ADP ribosylation factor binding protein GGA 3 antibody
    • ADP ribosylation factor binding protein GGA3 antibody
    • ADP-ribosylation factor-binding protein GGA3 antibody
    • ARF binding protein GGA 3 antibody
    • ARF binding protein GGA3 antibody
    • ARF-binding protein 3 antibody
    • gamma ear-containing antibody
    • GGA 3 antibody
    • GGA3 antibody
    • GGA3_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
    • Golgi localized gamma ear containing ARF binding protein 3 antibody
    • Golgi-localized antibody
    • KIAA0154 antibody
    see all

画像

  • Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg

    Secondary
    IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

    Predicted band size : 78 kDa

参考文献

This product has been referenced in:
  • Koike MA  et al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB ; Mouse . Read more (PubMed: 20472896) »
  • Pak Y  et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). Electron Microscopy ; Human . Read more (PubMed: 17116753) »

See all 2 Publications for this product

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