Anti-GGA2 抗体 (ab10552)
Key features and details
- Rabbit polyclonal to GGA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-GGA2 antibody
GGA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GGA2 -
由来種
Rabbit -
特異性
This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide:
LSMYRRPGQAPPDQEAL
conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).
|
特記事項 |
---|
WB
1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
-
機能
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
ドメイン
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
翻訳後修飾
Ubiquitinated. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 460336 Human
- Unigene: 29619 Mouse
-
別名
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
画像
-
Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary antibody anti-rabbit HRP ab6721 at 1/5000.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab10552 は 3 報の論文で使用されています。
- Duffney LJ et al. Epigenetics and autism spectrum disorder: A report of an autism case with mutation in H1 linker histone HIST1H1E and literature review. Am J Med Genet B Neuropsychiatr Genet 177:426-433 (2018). PubMed: 29704315
- Herskowitz JH et al. GGA1-mediated endocytic traffic of LR11/SorLA alters APP intracellular distribution and amyloid-ß production. Mol Biol Cell 23:2645-57 (2012). WB, ICC/IF ; Human . PubMed: 22621900
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . PubMed: 17666033