製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GGA1
  • 特異性
    The immunizing seqeunce is present only in isoform 1 of this protein, therefore cross-reactivity with splice variants is not expected.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    AMPTRPGEQASPEQPSA

    , corresponding to amino acids 336-352 of Human GGA1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ELISA 1/10000 - 1/50000.

ターゲット情報

  • 機能
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • ドメイン
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • 翻訳後修飾
    Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • 細胞内局在
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4930406E12Rik antibody
    • ADP ribosylation factor binding protein 1 antibody
    • ADP ribosylation factor binding protein GGA1 antibody
    • ADP-ribosylation factor-binding protein GGA1 antibody
    • ARF binding protein 1 antibody
    • ARF-binding protein 1 antibody
    • Gamma adaptin related protein 1 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 1 antibody
    • GGA 1 antibody
    • GGA1 antibody
    • GGA1 protein antibody
    • GGA1_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 1 antibody
    • Golgi localized gamma ear containing ARF binding protein 1 antibody
    • Golgi-localized antibody
    • OTTHUMP00000028975 antibody
    • OTTHUMP00000042200 antibody
    see all

画像

  • Anti-GGA1 antibody (ab10551) at 1/1350 dilution + Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/ml

    Secondary
    Goat-anti Rabbit IgG at 10000

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . Read more (PubMed: 17666033) »
  • Wahle T  et al. GGA1 is expressed in the human brain and affects the generation of amyloid beta-peptide. J Neurosci 26:12838-46 (2006). WB ; Human . Read more (PubMed: 17151287) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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