製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GFR alpha 3
  • アプリケーション
    適用あり: IHC (PFA fixed), IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 347-360 of Mouse GFR alpha 3.

  • ポジティブ・コントロール
    • Murine kidney, liver or heart tissue lysates.
  • 特記事項


    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed) 1/50.
IHC-P Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3 peptide (ab6100).

ターゲット情報

  • 機能
    Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.
  • 組織特異性
    Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
  • 配列類似性
    Belongs to the GDNFR family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GDNF family receptor alpha 3 antibody
    • GDNF family receptor alpha-3 antibody
    • GDNF receptor alpha-3 antibody
    • GDNFR-alpha-3 antibody
    • GDNFR3 antibody
    • GFR-alpha-3 antibody
    • GFRA3 antibody
    • GFRA3_HUMAN antibody
    • Glial cell line derived neurotrophic factor receptor alpha 3 antibody
    • GPI linked receptor antibody
    • OTTHUMP00000159486 antibody
    • OTTHUMP00000224020 antibody
    see all

画像

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3 with Anti-GFR alpha 3 antibody (ab8028) at 5μg/ml.
  • All lanes : Anti-GFR alpha 3 antibody (ab8028) at 1/500 dilution

    Lane 1 : Crude cell membrane fractions of murine heart
    Lane 2 : Crude cell membrane fractions of murine spleen
    Lane 3 : Crude cell membrane fractions of murine kidney
    Lane 4 : Crude cell membrane fractions of murine liver
    Lane 5 : Crude cell membrane fractions of murine brain


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Western blot analysis of GFR alpha 3 in crude cell membrane fractions of
    murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution.
  • ab8028 at 10µg/ml staining GFR alpha 3 in mouse heart tissue by IHC-P.

  • Immunohistochemical analysis of cleavage-stage Human embryo, staining GFR alpha 3 (green) with ab8028.

    Embyros were fixed with 4% paraformaldehyde for 30 min at 23°C. After permeabilization with 0.1% Triton X-100, embryos were pre-incubated in 5% BSA for 1 hour before incubation with primary antibody (diluted in PBS with 1% BSA) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Kawamura K  et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . Read more (PubMed: 23152897) »
  • Ceyhan GO  et al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P ; Human . Read more (PubMed: 16858191) »

See all 3 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Mouse Cell (Dorsal Root Ganglion (DRG) Cells E12)
Permeabilization
Yes - Saponin
Gating Strategy
1. FSC-A/SSC-A (exclusion of cell debris), 2. SSC-W (exclusion of cell doublets)
Specification
Dorsal Root Ganglion (DRG) Cells E12
Preparation
Cell harvesting/tissue preparation method: Trypsinization. Trituration.
Sample buffer: PBS BSA 1%
Fixation
Paraformaldehyde
Username

Georg Haase

Verified customer

投稿 Oct 14 2015

Thank you for your recent enquiry. Unfortunately, this antibody has not been tested for cross-reactivity with other family members. We apologise for the inconvenience caused, Please do not hesitate to contact us again if you need further inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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