製品の概要

  • 製品名Anti-GFP antibody
    GFP 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GFP
  • 特異性Anti-GFP assayed by ELISA for direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP.
  • アプリケーション適用あり: WB, IP, ELISA, IHC-P, IHC-FrFl, IHC-Fr, IHC - Wholemountmore details
  • 免疫原

    Fusion protein corresponding to Aequorea victoria GFP aa 1-246.
    Database link: P42212

  • ポジティブ・コントロール
    • HeLa cells, TGN mouse liver, E5.5 Hex-GFP transgenic mouse embryo, MRC5VA lung fibroblast whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/400 - 1/2000.

(for immunoprecipitated GFP, see Abreview).

IP Use at an assay dependent concentration.
ELISA 1/40000.

This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.

IHC-P 1/200 - 1/1000.
IHC-FrFl Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/1000.
IHC - Wholemount Use at an assay dependent concentration.

ターゲット情報

  • 関連性Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 別名
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Anti-GFP antibody 画像

  • Immunocytochemistry/ Immunofluorescence of TGN mouse liver labeling GFP on hepatocytes with ab6673.

  • Immunocytochemistry/ Immunofluorescence of E5.5 Hex-GFP transgenic mouse embryo labeling GFP with ab6673 at 1/500. Secondary antibody: Fluorchrome conjugated anti-goat IgG (1/10000) for 45 mins at room temperature. Staining GFP as green fluorescent signal with DAPI blue counterstain.

  • All lanes : Anti-GFP antibody (ab6673) at 1 µg/ml

    Lane 1 : HeLa cells
    Lane 2 : mock transfected HeLa cell lysate

    Lysates/proteins at 35 µg per lane.


    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-GFP antibody (ab6673) at 1/1000 dilution

    Lane 1 : MRC5VA lung fibroblast whole cell lysate overexpressing EGFP alone
    Lane 2 : MRC5VA lung fibroblast whole cell lysate overexpressing an EGFP fusion protein
    Lane 3 : MRC5VA lung fibroblast whole cell lysate overexpressing an EGFP fusion protein

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated anti-goat polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 27,55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous abreview.

    See Abreview

  • Immunohistochemistry of GFP transgenic mouse liver labeling GFP with ab6673. 

Anti-GFP antibody (ab6673) 使用論文

This product has been referenced in:
  • Nishio M  et al. Dysregulated YAP1/TAZ and TGF-ß signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A 113:E71-80 (2016). IHC-P . Read more (PubMed: 26699479) »
  • Ferreira MJ  et al. Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice. Cell Mol Gastroenterol Hepatol 1:550-569 (2015). IHC-P . Read more (PubMed: 26740970) »

See all 70 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample Mouse Tissue sections (spleen, red pulp, hematopoietic tissue)
Specification spleen, red pulp, hematopoietic tissue
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 01 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain, Neural Stem Cells)
Specification Brain, Neural Stem Cells
Permeabilization No
Fixative Paraformaldehyde
Username

Miss. Katya Zelentsova

Verified customer

投稿 Feb 26 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (GFP labelled 4T1 cells in the lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph9
Permeabilization No
Specification GFP labelled 4T1 cells in the lung
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. VIctoria Thompson

Verified customer

投稿 May 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (esophagus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citric acid, pH6.0
Specification esophagus
Blocking step Non-Abcam blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde
Username

Ms. Tatiana Karakasheva

Verified customer

投稿 Feb 22 2016

This anti GFP antibody has reacted with all tested variants of GFP from A. Victoria tested including YFP. We have not tested with eYFP specifically. The lots we have tested have not shown cross reactivity to RFP.

Application IHC - Wholemount
Sample Mouse Tissue (Epidermal sheet of the skin)
Specification Epidermal sheet of the skin
Username

Miss. yaara tabib

Verified customer

投稿 Jun 19 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Mouse Tissue sections (whole mouse embryo)
Specification whole mouse embryo
Permeabilization Yes - 0.1% Triton-X 100 in TBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 31 2014

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Triton X-100 (0.3%) in blocking buffer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2012

Gracias por contactarnos.

El anticuerpo ab6673 se ha testada en inmunofluorescencia con tejidos, bien sea congelados, o embebidos en parafina. No tenemos constancia de su uso en células, por lo que si esta es la aplicación que queréis llevar ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"