製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to GFP
  • 特異性
    detects GFP
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 免疫原

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein L purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab101863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 27 kDa.
IHC-P 1/500.

ターゲット情報

  • 関連性
    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 別名
    • GFP antibody
    • Green fluorescent protein antibody

Anti-GFP antibody 画像

  • Anti-GFP antibody (ab101863) at 1/5000 dilution + transgenic mice expressing the GFP gene product with IgY blocking reagent

    Secondary
    HRP-labeled goat anti-chicken antibodies

    Predicted band size : 27 kDa
  • ab101863, at a 1/500 dilution, staining GFP in transgenic mice expressing the GFP gene product by Immunohistochemistry.

Anti-GFP antibody (ab101863) 使用論文

This product has been referenced in:
  • Zeng X & Hou SX Enteroendocrine cells are generated from stem cells through a distinct progenitor in the adult Drosophila posterior midgut. Development 142:644-53 (2015). Read more (PubMed: 25670791) »

See 1 Publication for this product

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At Abcam, providing quality service is just as important as providing quality products. Lack of references does not mean the antibody has not been tested. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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