Anti-GFM1 抗体 [EPR12263-33] (ab173529)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12263-33] to GFM1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GFM1 antibody [EPR12263-33]
GFM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12263-33] to GFM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment within Human GFM1. The exact sequence is proprietary.
Database link: Q96RP9 -
ポジティブ・コントロール
- HeLa, NCI-H460, U87-MG and 293T cell lysates. HeLa cells. Immunoprecipitation pellet from HeLa whole cell lysate (ab150035).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12263-33 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173529の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
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IP |
1/10 - 1/100.
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ICC/IF |
1/50 - 1/100.
|
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 83 kDa. |
IP
1/10 - 1/100. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. -
パスウェイ
Protein biosynthesis; polypeptide chain elongation. -
関連疾患
Defects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy. -
配列類似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 85476 Human
- Entrez Gene: 28030 Mouse
- Entrez Gene: 114017 Rat
- Omim: 606639 Human
- SwissProt: Q96RP9 Human
- SwissProt: Q8K0D5 Mouse
- SwissProt: Q07803 Rat
- Unigene: 518355 Human
see all -
別名
- COXPD1 antibody
- EF-Gmt antibody
- EFG antibody
see all
画像
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All lanes : Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate: ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab173529 は 4 報の論文で使用されています。
- Molina-Berenguer M et al. Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations. FASEB J 36:e22091 (2022). PubMed: 34919756
- Bravo-Alonso I et al. Genes and Variants Underlying Human Congenital Lactic Acidosis-From Genetics to Personalized Treatment. J Clin Med 8:N/A (2019). PubMed: 31683770
- Yokokawa T et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). PubMed: 30369085
- Brito S et al. Long-term survival in a child with severe encephalopathy, multiple respiratory chain deficiency and GFM1 mutations. Front Genet 6:102 (2015). WB . PubMed: 25852744