Anti-GFAP (phospho S8) 抗体 [YC10] (ab115898)

製品の概要

  • 製品名Anti-GFAP (phospho S8) antibody [YC10]
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [YC10] to GFAP (phospho S8)
  • アプリケーション適用あり: ELISA, Functional Studies, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to the N-terminus of Pig GFAP phosphorylated at Ser8.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Functional Studies Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性Expressed in cells lacking fibronectin.
  • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylated by PKN1.
  • 細胞内局在Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
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    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
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    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP (phospho S8) antibody [YC10] (ab115898) 使用論文

This product has been referenced in:
  • Matsuoka Y  et al. Two different protein kinases act on a different time schedule as glial filament kinases during mitosis. EMBO J 11:2895-902 (1992). WB, ICC ; Human . Read more (PubMed: 1379174) »
  • Yano T  et al. A monoclonal antibody to the phosphorylated form of glial fibrillary acidic protein: application to a non-radioactive method for measuring protein kinase activities. Biochem Biophys Res Commun 175:1144-51 (1991). Read more (PubMed: 2025246) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call. Unfortunately, the blocking peptide for this antibodywill not beavailable for purchase through Abcam. It is possible that you may be able to find a porcine GFAP peptide phosphorylated at serine 8 by searching biocompare.c...

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Application IHC - Wholemount
Sample Mouse Tissue (myenteric plexus)
Specification myenteric plexus
Username

Dr. Ben Lee

Verified customer

投稿 Nov 21 2013

Thank you for your phone call. The sequence for the Porcine GFAP protein surrounding the phosphorylation site recognized by ab115898 is: MERRRVTS(p)AARRSYVSSLET. I hope this helps, please let me know if you need any other information.

Thank you for contacting Abcam earlier today.

I am sorry about the problems you have been having with ab115898 in western blotting. As we discussed on the telephone today I am going to send you a new aliquot of the antibody to test out. Your ...

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Thank you for contacting us. We are not aware of any immunohistochemistry data for this clone, YC10. I have added a reference to the datasheet form the laboratory that developed the clone in which staining of cell cultures (immunocytochemistry) is d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"