製品の概要

  • 製品名Anti-GFAP antibody [GF-02]
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GF-02] to GFAP
  • 特異性This antibody exclusively reacts with intact GFAP molecules.
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human, Pig
  • 免疫原

    Pellet of pig brain cold-stable proteins after depolymerization of microtubules (Pig).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
WB
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    • 組織特異性Expressed in cells lacking fibronectin.
    • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylated by PKN1.
    • 細胞内局在Cytoplasm. Associated with intermediate filaments.
    • Information by UniProt
    • 参照データベース
    • 別名
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      see all

    Anti-GFAP antibody [GF-02] 画像

    • ICC/IF image of ab21837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-GFAP antibody [GF-02] (ab21837) 使用論文

    ab21837 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"