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Purified GFAP from pig spinal cord
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) conjugated to Cy3®. The Cy3®-antibody conjugate is extensively dialyzed to remove unbound Cy3®.
Our Abpromise guarantee covers the use of ab49874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/400. The epitope recognized by the antibody is partially sensitive to prolonged formalin fixation, but is resistant to alcohol-fixation followed by paraffin-embedding and to acetone-fixation of frozen sections.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 20211723|
Immunocytochemistry/Immunofluorescence analysis of chicken astrocytes labelling GFAP (red) with ab49874. Nuclei stained using DAPI (blue). Cells were fixed with methanol for 15 minutes, washed in PBS and permeabilized with PBST for 10 minutes. Cells were then washed and blocked using 1% BSA in PBST for 30 minutes. Cells were incubated with the primary antibody (1:400 in blocking solution) for 1 hour at room temperature or overnight in a humid chamber in 4°C.