製品の概要

  • 製品名Anti-GFAP antibody [EPR1034Y]
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1034Y] to GFAP
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GFAP aa 1 to the C-terminus (N terminal).

  • ポジティブ・コントロール
    • WB: Human, Mouse and Rat brain tissue lysate; Human, Mouse and Rat cerebullum tissue lysate; IHC-P: Mouse brain, cerebral cortex and liver tissue sections; Human brain, cerebral cortex, hippocampus, colon and glioma tissue sections ICC/IF: Mouse cerebellum IP: Rat brain whole cell lysate
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

For unpurified use at 1/50 000 - 1/100 000.

IP 1/20 - 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性Expressed in cells lacking fibronectin.
  • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylated by PKN1.
  • 細胞内局在Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [EPR1034Y] 画像

  • IHC image of GFAP staining in a formalin fixed, paraffin embedded normal human hippocampus tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68428 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/Immunofluorescence analysis of mouse cerebellum tissue lysate labelling GFAP with ab68428 at 1/100. The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Antigen retrieval was performed by heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Nuclei were counterstained with DAPI (blue). PBS was used instead of the primary antibody as negative control.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human brain (left) and human glioma (right) tissue sections labelling GFAP with unpurified ab68428 at dilution of 1/250.

  • All lanes : Anti-GFAP antibody [EPR1034Y] (ab68428) at 1/10000 dilution

    Lane 1 : Human cerebellum tissue lysate at 20 µg
    Lane 2 : Human brain tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 48-50 kDa (why is the actual band size different from the predicted?)
    Blocking and Diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue sections labelling GFAP with purified ab68428 at a dilution of 1/500. The secondary antibody used was ab97051, Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • All lanes : Anti-GFAP antibody [EPR1034Y] (ab68428) at 1/10000 dilution

    Lane 1 : Mouse cerebellum tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Blocking and Diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue sections labelling GFAP with purified ab68428 at a dilution of 1/500. The secondary antibody used was ab97051, Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • All lanes : Anti-GFAP antibody [EPR1034Y] (ab68428) at 1/50000 dilution

    Lane 1 : Rat cerebellum tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Blocking and Diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of paraffin-embedded human colon tissue sections labelling GFAP with purified ab68428 at a dilution of 1/500. The secondary antibody used was ab97051, Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. 

  • ab68428 at 1/20 dilution immunoprecipitating GFAP in rat brain whole cell lysate observed at 50 KDa (lanes 1 and 2).

    Lane 1 (input): Rat brain whole cell lysate 10ug

    Lane 2 (+): ab68428 + Rat brain whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68428 in Rat brain whole cell lysate

    For western blotting, ab68428 was used followed by VeriBlot for IP (HRP) (ab131366) as the secondary antibody at a dilution of 1/10,000. 

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • All lanes : Anti-GFAP antibody [EPR1034Y] (ab68428) at 1/5000 dilution (unpurified)

    Lane 1 : Human brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue sections labelling GFAP with purified ab68428 at a dilution of 1/500. The secondary antibody used was ab97051, Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse brain tissue section labelling GFAP with unpurified ab68428 at dilution of 1/250.

Anti-GFAP antibody [EPR1034Y] (ab68428) 使用論文

This product has been referenced in:
  • FitzGerald P  et al. Expression of the type VI intermediate filament proteins CP49 and filensin in the mouse lens epithelium. Mol Vis 22:970-89 (2016). Read more (PubMed: 27559293) »
  • Wassink G  et al. Partial white and grey matter protection with prolonged infusion of recombinant human erythropoietin after asphyxia in preterm fetal sheep. J Cereb Blood Flow Metab N/A:N/A (2016). IHC . Read more (PubMed: 27207167) »

See all 5 Publications for this product

Product Wall

the dilution of ab68428 was 1/500.

Vielen Dank für Ihre Anfrage.

Laut Datenblatt muss dieser Antikörper bei -20C gelagert werden, und hat auch dann nur eine Lebensdauer von 12 Monaten. Normalerweise sind Antikörper relativ stabile Proteine, wenn sie den aufgereinigt wurden. D...

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Thank you for contacting Abcam.
The concentration for ab68428, lot GR18832-6, is ***** mg/mL.

Please let me know if there is anything else I can help you with.

We use Falcon, 96 well imaging plates, plastic bottom, PDL coated, from BD Biosciences for ELISA experiments are. Additionally, we have found that glass bottomed 96 well plates can give a lot of autofluorescence. In the future it would be best to avoid...

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