製品の概要

  • 製品名Anti-GFAP antibody
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to GFAP
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    H-CKLADVYQAELRELRLRLDQ-OH

    conjugated to KLH, corresponding to amino acids in GFAP. This sequence is conseverd in Human Mouse and Rat.

  • ポジティブ・コントロール
    • IHC-P and ICC/IF: Human and Rat brain tissue WB: Human brain homogenate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
ICC/IF
  • 追加情報ELISA: 1/100 - 1/10000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/4000 - 1/8000.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    • 組織特異性Expressed in cells lacking fibronectin.
    • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylated by PKN1.
    • 細胞内局在Cytoplasm. Associated with intermediate filaments.
    • Information by UniProt
    • 参照データベース
    • 別名
      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    Anti-GFAP antibody 画像

    • ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control: 40X objective.
    • ab90601, at a 1/6000 dilution, staining GFAP in Rat brain tissue (left image) by Immunohistochemistry. Right image shows negative control, 40X objective.
    • ab90601, at a 1/500 dilution, staining GFAP in Human brain tissue by Immunofluorescence. 40X objective.
    • ab90601, at a 1/500 dilution, staining GFAP in Rat brain by Immunofluorescence. 40X objective.
    • Anti-GFAP antibody (ab90601) at 1/6000 dilution + Human brain homogenate at 5 µg

      Predicted band size : 50 kDa

    Anti-GFAP antibody (ab90601) 使用論文

    ab90601 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your message and for providing this further information and the images.

    Reviewing the details, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note ...

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    Thank you for your reply.

    I look forward to receiving the images, I appreciate your cooperation.

    I would appreciate if you are also able to provide further information regarding the secondary antibodies, as previously requested. T...

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    Thank you for your reply and for kindly providing this further information.

    Reviewing the details, I fully understand your concerns and itregrettable the results fromthese three antibodies have not been satisfactory. It is unusual for 3 anti...

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    Thank you for your patience. My colleague is still out of office this week.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been repor...

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    Thank you for your email.

    Fixing in paraformaldehyde for more than 10-15 min will cross link the proteins to the point where antigen retrieval may be required to ensure the antibody has free access to bind and detect the protein. There is also...

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    Thank you for your email and I am sorry to hear about the problem you are experiencing.

    The protocol seems to fine to me, which is pretty much straight forward so the problem might be with the negative control cells you have chosen; these migh...

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    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ou...

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    Thank you for your rsponse.

    I have attached the protocol used by the lab for this product.

    Please let me know if you have any questions.

    Thank you for your enquiry.

    Sorry to hear this antibody is giving you trouble.

    A couple questions:

    1. What species of brain are present on the microarray?

    2. Have you tried using a more sensitive detection system...

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    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"