製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to GFAP
  • アプリケーション
    適用あり: IHC-FrFl, WB, Sandwich ELISA, IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human GFAP aa 417-430 (C terminal) (Cysteine residue).
    Sequence:

    DGEVIKESKQEHKD

  • ポジティブ・コントロール
    • WB: Mouse brain tissue lysate. IHC-P: Human cerebellum tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl 1/500 - 1/2000.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21943601

ターゲット情報

  • 機能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性
    Expressed in cells lacking fibronectin.
  • 関連疾患
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    Phosphorylated by PKN1.
  • 細胞内局在
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

画像

  • Anti-GFAP antibody (ab53554) at 0.1 µg/ml + Mouse Brain Lysate in RIPA Buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Primary incubation was 1 hour.
  • Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling GFAP with ab53554 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus.

    See Abreview

  • ab53554 staining GFAP in Mongolian gerbil brain tissue sections by Immunohistochemistry - Free Floating.Samples were incubated with ab53554 at a 1/500 dilution in 1% BSA, 0.3% Triton X-100, 0.1% Saponin for 16 hours at 4°C. ab6566 Donkey polyclonal Secondary Antibody to anti-goat Cy5 ® (IgG - H&L)), pre-adsorbed was used as the secondary antibody at a 1/100 dilution (green). Nuclei are counterstained with DAPI.

    See Abreview

  • ab53554 (5ug/ml) as the reporter with a capture rabbit antibody (5ug/ml).

参考文献

This product has been referenced in:
  • Arentsen T  et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017). IHC-FoFr ; Mouse . Read more (PubMed: 27843150) »
  • Onoda A  et al. Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle. Part Fibre Toxicol 14:4 (2017). IF ; Mouse . Read more (PubMed: 28148272) »

See all 49 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Permeabilization
Yes - 1% Triton-X
Specification
brain
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Formalin
Username

Abcam user community

Verified customer

投稿 Dec 06 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

投稿 Jun 13 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative
Formaldehyde
Username

Miss. Regina Vontell

Verified customer

投稿 May 01 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Zamboni
Username

Mr. Duc Dung Le

Verified customer

投稿 Apr 20 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Tissue lysate - other (Frontal Cortex)
Specification
Frontal Cortex
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification
total larvae, 4 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

投稿 Oct 27 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Rat Tissue sections (Nervous Tissue (Retina and Brain))
Specification
Nervous Tissue (Retina and Brain)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Retina)
Specification
Retina
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cell (Brain Organotypic Slice)
Specification
Brain Organotypic Slice
Permeabilization
Yes - 0.3% Triton
Fixative
Paraformaldehyde
Username

Ms. Kirsty Ireland

Verified customer

投稿 Dec 06 2013

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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