製品の概要

  • 製品名Anti-GFAP antibody
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to GFAP
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant GFAP Protein (Human)

  • ポジティブ・コントロール
    • e18 mouse brain This antibody gave a positive result in IHC in the following FFPE tissue: Human Hippocampus. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性Expressed in cells lacking fibronectin.
  • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylated by PKN1.
  • 細胞内局在Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
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Anti-GFAP antibody 画像

  • ICC/IF image of ab50738 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50738 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of GFAP staining in Human Hippocampus formalin-fixed paraffin-embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50738, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GFAP antibody (ab50738) 使用論文

This product has been referenced in:
  • Kelly T  et al. Ammonium-evoked alterations in intracellular sodium and pH reduce glial glutamate transport activity. Glia 57:921-34 (2009). Read more (PubMed: 19053055) »

See 1 Publication for this product

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