製品の概要

  • 製品名Anti-GFAP antibody
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GFAP
  • 特異性ab116010 distinguishes neoplasm of astrocytes origin from other neoplasm of the CNS.
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Human, Pig
  • 免疫原

    Purified Human GFAP protein.

  • ポジティブ・コントロール
    • Human brain or astrocytoma tissues. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
ICC 1/50 - 1/200.
ICC/IF 1/1000.

ターゲット情報

  • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性Expressed in cells lacking fibronectin.
  • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylated by PKN1.
  • 細胞内局在Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody 画像

  • ICC/IF image of ab116010 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116010 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GFAP antibody (ab116010) 使用論文

ab116010 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116010.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"