Anti-GFAP 抗体 [6F2] (ab8975)

製品の概要

  • 製品名Anti-GFAP antibody [6F2]
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6F2] to GFAP
  • 特異性Reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.
  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Glial fibrillary acidic protein (full length) from human brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/100 - 1/1000.

ターゲット情報

  • 機能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性Expressed in cells lacking fibronectin.
  • 関連疾患Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylated by PKN1.
  • 細胞内局在Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [6F2] 画像

  • GFAP immunogold labelling TEM of corticobasal degeneration brain tissue showing heavy and highly-specific labelling over a glial process. Bar =
    100 nm.

    This picture was kindly supplied as part of the review submitted by Dr Julian Thorpe (University of Sussex).

Anti-GFAP antibody [6F2] (ab8975) 使用論文

ab8975 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. For ab8975, the antibody was prepared by immunizing with a GFAP preparation containing the full length protein. You also enquired about ab7806 - the epitope has not been mapped. Please contact us again if you have any add...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (cultured neurones)
Specification cultured neurones
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

投稿 Dec 20 2005

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (rat astrocyte culture)
Specification rat astrocyte culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

投稿 Dec 20 2005

We can recommend to try a dilution of 1/100 but please remember to try a range of dilutions in a preliminary experiment to determine what dilution is best suited for your samples. You may also find the following reference useful: 1. Van Muijen, G. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"