製品の概要

  • 製品名
    Anti-GFAP antibody [2A5]
    GFAP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A5] to GFAP
  • アプリケーション
    適用あり: Sandwich ELISA, ICC, IHC-P, ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
    交差が予測される動物種: Mammal非交差種: Chicken
  • 免疫原

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • ポジティブ・コントロール
    • Homogenized cow, pig, human, rat, mouse or other mammalian brain extract in 1%SDS, 6M urea.
  • 特記事項

    This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA 1/5000.

Use this antibody as Capture antibody with Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) as Detection antibody at 0.5µg/ml.

ICC Use at an assay dependent concentration.
IHC-P 1/2000.
ICC/IF 1/10 - 1/50.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 組織特異性
    Expressed in cells lacking fibronectin.
  • 関連疾患
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    Phosphorylated by PKN1.
  • 細胞内局在
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [2A5] 画像

  • ab4648 staining GFAP - Astrocyte Marker in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody 1/2000 (TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to mouse IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.

    See Abreview

  • Rat neuron/glia cultures stained with nmouse monoclonal to GFAP ab 4648 (red).
  • Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Standard Curve for GFAP; dilution range 1 pg/ml to 1 µg/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 1/5000 and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 µg/ml.

  • Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

Anti-GFAP antibody [2A5] (ab4648) 使用論文

This product has been referenced in:
  • Hurtado-Alvarado G  et al. A2A Adenosine Receptor Antagonism Reverts the Blood-Brain Barrier Dysfunction Induced by Sleep Restriction. PLoS One 11:e0167236 (2016). WB, IHC-P ; Rat . Read more (PubMed: 27893847) »
  • Maekawa T  et al. Leucine-rich repeat kinase 2 (LRRK2) regulates a-synuclein clearance in microglia. BMC Neurosci 17:77 (2016). ICC/IF ; Mouse . Read more (PubMed: 27903237) »

See all 23 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
spinal cord
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Chia-Li Lin

Verified customer

投稿 Nov 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RTP°C
Sample
Mouse Cell (Mouse primary astrocyte)
Specification
Mouse primary astrocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Craig Beall

Verified customer

投稿 Sep 15 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Embryonic rat cerebral cortex)
Specification
Embryonic rat cerebral cortex
Fixative
Paraformaldehyde
Permeabilization
Yes - Ice cold methanol
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 26 2012

Thank you for contacting Abcam.

Earlier we discussed your sELISA with ab4648 as the capture and ab48050 as the detector. As we discussed, since you would like to use your streptavidin-gold conjugated secondary, a detector that is conjugated to...

Read More

The protocol used was as follows:
Buffers and Reagents:
Carbonate Coating Buffer (100 mM)
Antigen or antibody should be diluted in coating buffer to immobilize them to the wells:
3.03 g Na2CO3,
6.0 g NaHCO3
Make up to 1000...

Read More

We do recommend usingthe Sodium Citrate buffer (pH 6.0), however HIER are effective as well.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Username

Mr. Marian Hruska-Plochan

Verified customer

投稿 Feb 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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