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Synthetic peptide within residues: RGCIGEAWAK DSGLVRCPEC NQAYNQKPGL EKNLKLTNIV EKFNALHVEK , corresponding to N terminal amino acids 35-84 of Human GERP (NP_112174)
Our Abpromise guarantee covers the use of ab83395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GERP with ab83395 at 5.0µg/ml.
ab83395 has not yet been referenced specifically in any publications.