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Tissue, cells or virus corresponding to Mouse Germ cell-specific antigen. Cell lysates of adult mouse testes.
Our Abpromise guarantee covers the use of ab82527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/5000. Predicted molecular weight: 146 kDa.|
ab82527 staining Germ cell-specific antigen in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150165 was used as the secondary antibody, undiluted.
ab82527 staining Germ cell-specific antigen in mouse postnatal day 7 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 45 minutes at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA) for 1 hour at 25°C. A biotin-conjugated goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.