Anti-Germ cell-specific antigen 抗体 [TRA98] (ab82527)

製品の概要

  • 製品名
    Anti-Germ cell-specific antigen antibody [TRA98]
  • 製品の詳細
    Rat monoclonal [TRA98] to Germ cell-specific antigen
  • 特異性
    In PubMed: 27718356 the epitope of ab82527 has been mapped to a murine-specific 8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat that occurs 25 times in mouse GCNA1 (aa 167- 383).
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse Germ cell-specific antigen. Cell lysates of adult mouse testes.

  • ポジティブ・コントロール
    • Testicular germ cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent dilution.
WB 1/5000. Predicted molecular weight: 146 kDa.

ターゲット情報

  • 関連性
    Detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
  • 別名
    • GENA antibody
    • Testicular germ cell-specific antigen antibody

Anti-Germ cell-specific antigen antibody [TRA98] 画像

  • ab82527 staining Germ cell-specific antigen in mouse postnatal day 7 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 45 minutes at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA) for 1 hour at 25°C. A biotin-conjugated goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

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  • ab82527 staining Germ cell-specific antigen in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150165 was used as the secondary antibody, undiluted.

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  • All lanes : Anti-Germ cell-specific antigen antibody [TRA98] (ab82527) at 0.1 µg/ml

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 3 : mouse intestine lysate
    Lane 4 : mouse kidney lysate
    Lane 5 : mouse liver lysate
    Lane 6 : mouse lung lysate
    Lane 7 : mouse spleen lysate
    Lane 8 : mouse ovary lysate
    Lane 9 : mouse testis lysate

    Lysates/proteins at 100 µg per lane.


    Predicted band size : 146 kDa
    Western blot analysis of various mouse tissues, using ab82527.
    In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
  • Immunohistochemical analysis of mouse fetal ovary tissue, staining Germ cell-specific antigen with ab82527.

    Tissue was fixed with Bouin's fixative, permeabilized with 0.05% Tween 20 and 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA and 0.5% Triton X-100 in 1X PBS) for 1 hour at 25°C. An AlexaFluor®488-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

Anti-Germ cell-specific antigen antibody [TRA98] (ab82527) 使用論文

This product has been referenced in:
  • Magaraki A  et al. Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development. Epigenetics Chromatin 10:11 (2017). IF ; Mouse . Read more (PubMed: 28293300) »
  • Dumont L  et al. Vitamin A prevents round spermatid nuclear damage and promotes the production of motile sperm during in vitro maturation of vitrified pre-pubertal mouse testicular tissue. Mol Hum Reprod 22:819-832 (2016). Read more (PubMed: 27671755) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Apr 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (fibroblast)
Permeabilization
No
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
ice-cold acetone
Username

Abcam user community

Verified customer

投稿 Jul 07 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Sample
Mouse Tissue sections (Postnatal day 7 mouse testes)
Specification
Postnatal day 7 mouse testes
Permeabilization
No
Fixative
4% PFA
Username

Abcam user community

Verified customer

投稿 Dec 29 2014

Thank you for your inquiry and your patience with this reply.
For ab82527, it should be IgG2a, although we do not do any validation to verify that. The clone TRA98 recognizes a mouse testicular germ cell-specific antigen (1). In adult tissues, ban...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (fetal ovary)
Specification
fetal ovary
Fixative
Bouin's fixative
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization
Yes - 0.05% Tween 20 and 0.1% Triton X-100 in blocking serum
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2012

DISCOUNT CODE: ***
Expiration date: February 15th, 2012
Value: ***

I am very pleased to hear you would like to accept our offer and test ab82527 in IHC-P. This code will give you *** off your next order before the expiration date. To...

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Thank you very much for your email.
I am happy to arrange a credit note or refund. However I would need more information about the problem customer experienced. Our customers feedback and own tested data show that the antibody detects Germ cells v...

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Thank you very much for your valuable feedback.

We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us.

To find...

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Thank you for contacting us. To answer your questions: 1) Yes, JLP is the same as SPAG9. For more alternative names, please check the protein database entry for the mouse protein: http://www.uniprot.org/uniprot/Q58A65 2)References are listed in the ref...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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