Generic Caspase Activity Assay Kit (Fluorometric - Red) (ab112131)

製品の概要

  • 製品名Generic Caspase Activity Assay Kit (Fluorometric - Red)
    Caspase キット 製品一覧
  • テスト
    1 x 100 test
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD).

    ab112131 is designed to monitor cell apoptosis through measuring generic caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) activation in live cells with our red fluorescent TF5-VAD-FMK probe. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). The cell permeable and nontoxic TF5-VAD-FMK irreversibly binds to activated casepase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further activation but will not stop apoptosis from proceeding.

    ab112131 can be conveniently used with the common fluorescence instruments equipped with the light sources and filters Ex/Em = ~647/660 nm. The red label allows for direct detection of activated caspases in apoptotic cells by a flow cytometer. It can be used for the quantification of most activated caspases activities in apoptotic cells, or for the screening of caspases inhibitors. The kit provides all the essential components.

    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション適用あり: Flow Cyt, Functional Studiesmore details

製品の特性

  • 関連性Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • 細胞内局在Cytoplasmic

アプリケーション

Our Abpromise guarantee covers the use of ab112131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Generic Caspase Activity Assay Kit (Fluorometric - Red) 画像

  • Jurkat cells were untreated (Blue) or with 20 μM camptothecin (Pink) in a 37oC, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF5-VAD-FMK for 1 hour.

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Generic Caspase Activity Assay Kit (Fluorometric - Red) (ab112131) 使用論文

ab112131 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"